smartscribe 5× first strand buffer (TaKaRa)
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TaKaRa
smartscribe 5× first strand buffer
Smartscribe 5× First Strand Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse-transcriptase+buffer/SMARTScribe+Reverse+Transcriptase/pmc09901934-194-8-12
Average 96 stars, based on 1136 article reviews
Smartscribe 5× First Strand Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse-transcriptase+buffer/SMARTScribe+Reverse+Transcriptase/pmc09901934-194-8-12
Average 96 stars, based on 1136 article reviews
smartscribe 5× first strand buffer - by Bioz Stars,
2026-10
96/100 stars
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Concentration Assay:Article Title: A bispecific antibody designed to act as a NRP2/PLXNA1 agonist mimics anti-cancer activity of SEMA3F. Article Snippet: .. A 6.79 μL reaction mixture was prepared, containing a final concentration of 1 mM dNTPs, 2 mM DTT, 1x first-strand buffer, 5 U/μL Article Title: A bispecific antibody designed to act as a NRP2/PLXNA1 agonist mimics anticancer activity of SEMA3F Article Snippet: .. A 6.79 μl reaction mixture was prepared, containing a final concentration of 1 mM dNTPs, 2 mM DTT, 1x first-strand buffer, 5 U/μl Reverse Transcription:Article Title: A bispecific antibody designed to act as a NRP2/PLXNA1 agonist mimics anti-cancer activity of SEMA3F. Article Snippet: .. A 6.79 μL reaction mixture was prepared, containing a final concentration of 1 mM dNTPs, 2 mM DTT, 1x first-strand buffer, 5 U/μL Article Title: Drought Stress Inhibits the Accumulation of Rotenoids and the Biosynthesis of Drought-Responsive Phytohormones in Mirabilis himalaica (Edgew.) Heim Calli Article Snippet: .. Subsequently, 4 μL of 5× Article Title: scFFPE-ATAC enables high-throughput single cell chromatin accessibility profiling in formalin-fixed paraffin-embedded samples. Article Snippet: The reaction mixture (11.25μL), containing 500ng-1 μg of RNA, 1μL of 100μM FirstStrand_cDNA oligo, and 0.25μL of RNase Inhibitor (20U/μL, ThermoFisher Scientific, cat. no. N8080119), is heated at 70 °C for 3min, then immediately cooled on ice. .. Next, the master mix is added, which consists of: • 4μL of 5× Article Title: scFFPE-ATAC enables high-throughput single cell chromatin accessibility profiling in formalin-fixed paraffin-embedded samples Article Snippet: The reaction mixture (11.25 μL), containing 500ng-1 μg of RNA, 1 μL of 100 μM FirstStrand_cDNA oligo, and 0.25 μL of RNase Inhibitor (20 U/μL, Thermo Fisher Scientific, cat. no. N8080119), is heated at 70 °C for 3 min, then immediately cooled on ice. .. Next, the master mix is added, which consists of: 4 μL of 5× Article Title: Gene expression QTL mapping in stimulated iPSC-derived macrophages provides insights into common complex diseases Article Snippet: Using the automated Zephyr G3 NGS Workstation (Perkin Elmer), mRNA was purified from the cell lysates in 96 well plates using the mRNA DIRECT kit (Thermo Fisher, 61012), according to the manufacturer’s instructions, using 20 μL of oligo dT Dynabeads. .. 5 μL of Article Title: A bispecific antibody designed to act as a NRP2/PLXNA1 agonist mimics anticancer activity of SEMA3F Article Snippet: .. A 6.79 μl reaction mixture was prepared, containing a final concentration of 1 mM dNTPs, 2 mM DTT, 1x first-strand buffer, 5 U/μl other:Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators. Article Snippet: Five μL of RNA wasmixed with 0.5 μL of oKK145 [12 μM], incubated at 70 °C for 3min, put on ice to form RNA/primer complex and mixed with 4.5 μL of RT solution {0.5 μL of SMARTScribe RTase (cat. #639536, Takara), 2 μL of 5x first strand buffer (supplied with RTase), 0.25 μL of DTT (supplied with RTase), 1 μL of dNTPs (cat. #N0447, NEB), 0.5 μL of custom LNA-TSO [12 μM] (cat. # 339412, Qiagen) and 0.25 μl of Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators Article Snippet: Five μL of RNA was mixed with 0.5 μL of oKK145 [12 μM], incubated at 70 °C for 3 min, put on ice to form RNA/primer complex and mixed with 4.5 μL of RT solution {0.5 μL of SMARTScribe RTase (cat. #639536, Takara), 2 μL of 5x first strand buffer (supplied with RTase), 0.25 μL of DTT (supplied with RTase), 1 μL of dNTPs (cat. #N0447, NEB), 0.5 μL of custom LNA-TSO [12 μM] (cat. # 339412, Qiagen) and 0.25 μl of Incubation:Article Title: scFFPE-ATAC enables high-throughput single cell chromatin accessibility profiling in formalin-fixed paraffin-embedded samples. Article Snippet: The reaction mixture (11.25μL), containing 500ng-1 μg of RNA, 1μL of 100μM FirstStrand_cDNA oligo, and 0.25μL of RNase Inhibitor (20U/μL, ThermoFisher Scientific, cat. no. N8080119), is heated at 70 °C for 3min, then immediately cooled on ice. .. Next, the master mix is added, which consists of: • 4μL of 5× Article Title: scFFPE-ATAC enables high-throughput single cell chromatin accessibility profiling in formalin-fixed paraffin-embedded samples Article Snippet: The reaction mixture (11.25 μL), containing 500ng-1 μg of RNA, 1 μL of 100 μM FirstStrand_cDNA oligo, and 0.25 μL of RNase Inhibitor (20 U/μL, Thermo Fisher Scientific, cat. no. N8080119), is heated at 70 °C for 3 min, then immediately cooled on ice. .. Next, the master mix is added, which consists of: 4 μL of 5× |
